Application of Biotechnology in Water Treatment: Immobilization of Microorganisms by Inclusion

The work concentrated on a method for immobilizing microorganisms, including natural polymer-based support, a crucial tool in biotechnological processes. Bacteria have been immobilized in calcium alginate beads; the ideal gelification concentrations have been determined based on the stability of the beads concerning gel dissolution, cellular enlargement in the surrounding medium and nutrient diffusion issues. The number of live cells within the bead was monitored by dissolving the gel in a sterilized tampon with a pH of 7.