Rapid, Sensitive and Label-Free Multiplex Meat Speciation Kit

A label-free, one-step multiplex high-resolution melting (HRM) assay has been developed for the identification of five different meat species namely beef, pork, sheep, chicken, and duck. HRM involves PCR amplification of the region of interest in the presence of double-stranded DNA binding dye followed by melting at high resolution. The HRM assay is rapid, sensitive, cost-effective and allows simultaneous detection of multiple species in meat and meat products.