Antibacterial Activities and Efficacy of Carica Papaya Seed Extract on Fragaria x Ananassa (Strawberry)

This study examines the antibacterial properties, chemical composition, and effectiveness of the extract Carica papaya seed (PS) var. Sekaki/Hongkong. The Carica papaya seed was extracted using two different solvents (water and ethanol), and different methods of extraction; maceration-filtration (F) and maceration-centrifuge-filtration (C). The extracts were tested for antibacterial properties against eight Gram-positive and four Gram-negative microorganisms. The most potent extract was subsequently subjected to phenolic and flavonoid assays and an efficacy evaluation on actual food, i.e.Fragaria x ananassa (Strawberry). Carica papaya seed extract using water (PSW) showed moderate antibacterial activities against L. monocytogenes and S. aureus, while ethanolic extract (PSE) exhibited high antibacterial activities against all Gram-positive bacteria. Therefore, the PSE was chosen as the most effective extract to inhibit the tested bacteria. Filtered PSE (PSEF) inhibited B. cereus, C. diphtheria, L. monocytogenes and S. aureus with a minimum inhibitory concentration (MIC) between 2.813 – 5.625 mg/mL. The PSE's total phenolic (TPC) and total flavonoid content (TFC) ranged between 32.183 to 42.725 mg GAE/g DW and 0.118 to 0.142 mg QE/g DW, respectively. The optical density measuring method was used in this study to assess the efficacy of the PSEF against C. diphtheria, L. monocytogenes and S. aureus on Fragaria x ananassa (Strawberry) on refrigerated temperatures. The outcome showed that the PSEF could efficiently inhibit the tested microorganisms.