Extraction of Glucose Oxidase from Aspergillus Niger for Glucose Monitoring

Diabetes is progressively emerging as a significant health challenge in Algeria, impacting individuals across various age groups. Inadequate management of diabetes may give rise to complications, underscoring the importance of effective disease control and care. The expenses associated with monitoring glucose levels show a consistent year-over-year increase and following to the current situation of our country's national policy to stop importing products and replace them with local products, encouraging national production. In this context, we searched for the viability of employing Aspergillus niger as a feedstock for fermentation-based glucose oxidase (GOX) synthesis. The aim was to increase overall GOX activity through the selection of an available substrate and the cultivation of microorganisms. After 168 hours of fermentation, the enzyme produced 2.6 μmole min^-1 at a 10% glucose concentration. The ideal pH and temperature ranges for the synthesis of the enzyme were searched to be 5.0–6.0 and 30–35°C, respectively.