Beneficial Electroactive Biofilm for Sensing: Feasibility and Perspective

Objective of this work is to develop electroactive biofilms from various natural environments (soil and sediment).  The ultimate goal of studying behavior of EABs is to gain necessary information on engineering biofilms that can be powerful and efficient in field-scale applications, such as, biosensing. In this study chronoamperometry is an efficient technique to form electroactive biofilms (EAB) on conductive electrodes. The rhizospheric soil of CDER and sediment Dounia parc lac were analyzed as a potential inoculum to form MFC anodes with the use of acetate as substrate. Working electrodes (WE) polarized at +0.155 V/SCE gave better results compared to +0.3 V/SCE and -0.3 V/SCE. For soil the sediment test +0.2V/SCE was appropriate potential for bio-current generation.