Isolation and Biochemical Characterization of Cellulase Producing Goat Rumen Bacteria

Authors

Sharmin Sultana
Department of Biochemistry & Molecular Biology, Hajee Mohammed Danesh Science & Technology University, Dinajpur 5200, Bangladesh, National Institute of Biotechnology, Ganakbari, Ashulia, Savar, Dhaka, Bangladesh
Md. Sad Salabi Sawrav
Department of Biotechnology and Genetic Engineering, Noakhali Science and Technology University, Sonapur, University Rd, Noakhali
Snygdha Rani Das
Department of Biochemistry and Molecular Biology, Hajee Mohammed Danesh Science and Technology University, Dinajpur 5200
Mehfuz Alam
Department of Biochemistry and Molecular Biology, Hajee Mohammed Danesh Science and Technology University, Dinajpur 5200
Md. Abdul Aziz
Department of Biochemistry and Molecular Biology, Hajee Mohammed Danesh Science and Technology University, Dinajpur 5200
Md. Al-Amin Hossain
Department of Biochemistry and Molecular Biology, Hajee Mohammed Danesh Science and Technology University, Dinajpur 5200, Bangladesh
Md. Azizul Haque
Department of Biochemistry & Molecular Biology, Hajee Mohammed Danesh Science & Technology University, Dinajpur 5200

Synopsis

Cellulose is the most prevalent polymer on the planet and has long been utilized for a variety of industrial applications. The study's goal was to screen and isolate cellulase-producing bacteria from the rumen of a goat collected from different location of Dinajpur district. To do so, rumen content samples from two distinct goats were collected. In this investigation, rumen cellulase-producing bacteria were isolated and characterized after serial dilution of five isolates up to six fold and inoculation into Nutrient agar. Following that, all of the isolates were underwent Methyl Red (MR) test & Voges-Proskauer (VP) test to identify organism’s metabolic pathway, Triple Sugar Iron Agar (TSI) Test to determine bacterial ability to utilize sugar, Motility Indole and Urease activity test (MIU) to determine motility, Urease utilization and can produce Indole or not, Citrate utilization test to utilize citrate as carbon and energy source, Oxidase test, Catalase test to check the presence of catalytic enzyme. The result revealed the colonial characterization of bacteria and also where proven all five isolates are promising enough and superior in quality to produce cellulose.

ICETEAS-2021
Published
February 14, 2022
Online ISSN
2582-3922