A Potential Halal Collagen-like Protein (RecCLPM-46) Exhibits Wound Healing Properties for Halal Pharmaceutical Industry

Collagen is a powerful biomaterial that can be used in various industries, mainly, the medical and pharmaceutical industries. It is often used and incorporated to speed up the wound-healing process. A halalan toyyiban collagen uses for wound healing is in high demand for Muslims that abiding the Syariah law. In this study, the collagen-like protein, cloned from Methylobacteria sp. 4-46 (RecCLPM4-46) and purified using FPLC was tested for its wound healing property. The HaCaT keratinocyte skin cells were used to simulate the proliferation activity of the skin. Allantoin, the commercial wound healing aid in the market was used as the positive control, six different concentrations of RecCLPM4-46 (1.56 %, 3.13 %, 6.25 %, 12.50% and 25.0% v/v) were applied in the cell viability assay. Due to non-cytocompatibility, 12.50% and 25.0% v/v of RecCLPM4-46 were eliminated for wound closure assay. In the scratch assay, 6.25% v/v of RecCLPM4-46 has the highest wound closure percentage (65.4%) compared to the rest of the RecCLPM4-46 concentrations. In terms of wound closure percentage, 6.25% v/v of RecCLPM4-46 was not far off with 26.1% less wound closer from Allantoin. This result suggests that RecCLPM4-46 has a promising wound-healing feature for the biomedical and pharmaceutical industries.